Name :
NT5C3A/NT5C3 Protein
Description :
NT5C3A (5′-Nucleotidase, Cytosolic IIIA) is a Protein Coding gene. This gene encodes a member of the 5′-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5′-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5′ nucleotidase and catalyzes the dephosphorylation of pyrimidine 5′ monophosphates. NT5C3A expression required both an intronic IFN-stimulated response element and the IFN-stimulated transcription factor IRF1. Overexpression of NT5C3A, but not of its catalytic mutants, suppressed IL-8 production by HEK293 cells. NT5C3A-stimulated sirtuin activity resulted in deacetylation of histone H3 and the NF-kappaB subunit RelA (also known as p65), both of which were associated with the proximal region of the Il8 promoter, thus repressing the transcription of Il8 Together.
Species :
Human
Uniprotkb :
E. coli
Tag :
Tag Free
Synonyms :
P5N1, P5N-1, hUMP1, NT5C3, cN-III, PSN1, UMPH1, UMPH, POMP, 5′-nucleotidase, cytosolic IIIA, P5’N-1, PN-I, p36
Construction :
A DNA sequence encoding the mature form of human NT5C3 (NP_001002010) (Met 12-Leu 297) was expressed.
Protein Purity :
> 92 % as determined by SDS-PAGE
Molecular Weight :
Approxiamtely 32.7kDa
Endotoxin :
Please contact us for more information.
Formulatione :
Lyophilized from sterile PBS. Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
NT5C3A (5′-Nucleotidase, Cytosolic IIIA) is a Protein Coding gene. This gene encodes a member of the 5′-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5′-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5′ nucleotidase and catalyzes the dephosphorylation of pyrimidine 5′ monophosphates. NT5C3A expression required both an intronic IFN-stimulated response element and the IFN-stimulated transcription factor IRF1. Overexpression of NT5C3A, but not of its catalytic mutants, suppressed IL-8 production by HEK293 cells. NT5C3A-stimulated sirtuin activity resulted in deacetylation of histone H3 and the NF-kappaB subunit RelA (also known as p65), both of which were associated with the proximal region of the Il8 promoter, thus repressing the transcription of Il8 Together.
References and Literature :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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