Surface-enhanced Raman scattering (SERS) has emerged as a powerful analytical technique for ultrasensitive detection of biomolecules at the nanoscale. In this work, we developed a novel class of organelle-targeting SERS nanosensors capable of real-time, non-invasive monitoring of subcellular pH dynamics. The nanosensors are constructed by integrating pH-responsive Raman reporters with plasmonic gold nanoparticles functionalized with targeting ligands specific to intracellular organelles such as mitochondria, lysosomes, and endoplasmic reticulum. The design leverages the strong electromagnetic enhancement provided by localized surface plasmon resonance (LSPR) in gold nanostructures, combined with the chemical specificity of Raman spectroscopy, enabling precise quantification of pH changes within individual organelles. The pH-sensitive Raman probe is based on a molecular system whose vibrational modes shift predictably with protonation state, allowing for calibration-free, ratiometric analysis of pH variations. By conjugating these probes with organelle-specific peptides or antibodies, the nanosensors achieve selective accumulation within target compartments, minimizing background interference and enhancing spatial resolution. Confocal SERS imaging confirmed successful delivery and localization of the sensors inside living cells, with signal intensity correlated to local pH levels. The system demonstrated high sensitivity (detection limit < 0.1 pH unit), rapid response time (< 10 seconds), and excellent reproducibility across multiple cell lines. Moreover, the sensors maintained stability under physiological conditions and exhibited minimal cytotoxicity even after prolonged incubation. This platform was successfully applied to monitor dynamic pH fluctuations during cellular processes such as mitophagy, autophagy, and apoptosis, revealing previously unobserved pH gradients between different organelles.Dynamin II Antibody Formula These findings underscore the potential of targeted SERS nanosensors as a transformative tool for studying intracellular microenvironmental regulation, offering unprecedented insights into disease mechanisms related to pH dysregulation, including cancer, neurodegeneration, and metabolic disorders.CD33 Antibody Purity The modular design allows for easy adaptation to other analytes, making it a versatile platform for advanced biomedical diagnostics and live-cell imaging.PMID:34715300 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com