Fri. Jun 21st, 2024

The membranes were washed and incubated with secondary horseradish peroxidase-coupled antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) in TBS-T for 1 hour at area temperature. After the ultimate washes, the proteins had been detected by enhanced chemiluminescence

Cell apoptosis was assessed by The DeadEndTM Fluorometric TUNEL Method (Promega Madison, WI, Usa) following manufacturer's directions. forty nine,6-Diamidino-2-phenylindole (DAPI) (d9564) from Sigma-Aldrich was used to stain nuclei.Double-immuno fluorescence scientific…