Within and around G-rich regions (Table 2). Site-directed mutagenesis of Grepeat motifs was studied to determine their roles in HAS1 splicing. Mutagenized sequences for each motif are shown in Figure…
Total of 250 ng of mitochondrial protein in 50 mM NaHCO3 was coated on an enhanced proteinbinding ELISA plate (Caster) by incubating at 4uC for 8 h. Nonspecific binding to…
Ogen). Ten micrograms of total RNA were fractionated by electrophoresis on 1.3 agarose gels containing 2.2 M formaldehyde (Sigma) and blotted and hybridized as described previously .Generation of Expression Clones…
Responses.The APM consists of several intracellular proteins responsible for processing, transport and chaperoning of peptides derived mostly, but not exclusively, from endogenous proteins for crosspresentation. After cleavage of these proteins…