D between conditions. We aimed to detect non-linear effects of therapy with silimarin. We’ve detected octamer RNA sequences that are over-represented within the exosomes from stimulated cells and we’ve got utilized these motifs to detect mRNAs related to lipid metabolism that are a lot more most likely to become the targets of micro RNAs with these certain motifs. We validated our predictions making use of the readily available databases for miRNA-target interaction prediction (e.g. PITA, TargetScanS). We’ve got OTUB2 Proteins Recombinant Proteins employed Renyi Divergence of order 0.five to quantify the similarity of expression patterns of all transcripts, to a pre-selected set of miRNAs known to be involved in inflammatory pathway, across all experimental circumstances. We’ve got performed hierarchical clustering to detect co-regulated micro RNAs. Results: Amongst most drastically changed by inflammatory stimuli exosomal miRNAs had been: hsa-let-7b-5p, targeting IRS2, involved in steatohepatitis, hsa-miR-6790-5p and hsa-miR-146b-5p, whereas silimarin affected exosomal presence of hsa-miR-146b-5p, hsa-miR-542-3p. mir146b was reported to directly influence TRAF6 and IRAK1 mRNA and protein levels in macrophages Summary/Conclusion: We’ve got identified set of miRNAs, which presence in Hep2G exosomes is altered by inflammatory stimuli and which could potentially impact expression of genes involved in lipid metabolism in KCs. The precise influence of Hep2G-derived miRNA on KCs need additional investigation.LBP.Platelet derived-microparticles as modulator of plasmacytoid dendritic cell inflammatory response Adam Ceroi1, Sameh Obeid2, Thomas Cherrier3, C ine Elie-Caille2, Wilfried Boireau2 and Philippe SaasRavicahndran’s Lab., University of Virgina, VA, USA; 2Institut FEMTO-ST, CNRS, Univ. Bourgogne Franche Comte; 3INSERM UMR 1098, ESFBourgogne-Franche Comt Univ. de Franche-ComteLBP.Exosomal miRNA in Hep2G cells stimulated by pro-inflammatory cytokines Justyna Toto-uraska1, Michal Seweryn1, Katarzyna Poskrubek2, Anna Winiewska2, Rafal Olszanecki2, Pawel Wolkow3 and Ryszard Korbut1 Jagiellonian University Healthcare College Center for Health-related Genomics OMICRON, Krakow, Poland; 2Jagiellonian University Healthcare College Division of Pharmacology, Krakow, Poland; 3Jagiellonian University Health-related College Center for Health-related Genomics – OMICRON, Jagiellonian university Medical College Department Of Pharmacology, Krakow, Poland;Introduction: Microparticles (MP) are generated in the plasma membrane of parental cells just after Tissue Inhibitor of Metalloproteinase 4 (TIMP-4) Proteins Recombinant Proteins activation or cell death. According to the stimulus responsible for MP generation, it was demonstrated that the quantity, content material and biological activities of MP could differ. Previously, we demonstrated that platelet or endothelial cell-derived MP uptake by plasmacytoid dendritic cells (PDC) can modulate the Liver X Receptor (LXR) pathway, and then regulate inflammatory responses. Right here, we applied MP from resting- or collagen activated-platelet (rest-PMP or colPMP, respectively) to compare their size and protein content, and investigate their effect around the LXR pathway along with the subsequent inflammatory response in PDC. Approaches: Platelet from healthful donors have been stimulated or not by collagen, and platelet derived-MP (PMP) had been isolated by centrifugation. PMP size and concentration had been investigated by flow cytometry and Surface Plasmon Resonance coupled with Atomic Force Microscopy, followed by Mass Spectrometry to ask their protein content material. Applying PDC from wholesome donors, we investigated LXR pathway involvement (via LXR-target gene.