We hypothesized that the particular proteins containing EV released from stroke serum cultured MSCs may possibly affect the neurogenesis and angiogenesis of recipient cells. Solutions: EVs had been purified from conditioned media of MSC cultured with FBS (FBS-MSC) and MSC cultured with stroke serum (SS-MSC). These EVs had been characterized by nanoparticle tracking analysis. EV protein profiling in conditioned media was systematically compared via using LC-MS/MS-based label-free quantification. Real-time PCR was performed to ascertain the distinction in the gene expression in each cell. The protein concentration in the EV was confirmed by ELISA. Results: A total of 1068 proteins were identified from SS-MSC-EV and FBS-MSC-EV by way of LC-MS. According to statistical analysis, 22 proteins had been found to become much more than 2-fold (p 0.05) upregulated in SS-MSC_EV. ITGA5, CLU, and CTSB were significantly increased of N-type calcium channel Gene ID SS-MSC gene expression levels in comparison to FBS-MSC. Among the candidate proteins, clusterin (CLU) was identified to become upregulated in EVs from SS-MSC compared to those from FBS-MSC. Summary/Conclusion: These outcomes suggest that SSMSC_EVs containing clusterin may promote intercellular communication and have an effect on neurogenesis and angiogenesis of recipient cells. Funding: This study was supported by a grant from the Korean Healthcare Technology R D Project, Ministry of Overall health Welfare (HI17C1256) and Standard Science Study Plan, the Ministry of Science, ICT and Future Arranging (2018M3A9H1023675).PT10.Adipose-derived Stem/Stromal Cell secretome, containing both soluble components and extracellular vesicles, exerts chondroprotective effects in vitro Chiara Giannasia, Stefania Niadaa, Sara Casatib and Anna BrinicaPT10.Proteomic analysis of extracellular vesicles from MSC cultured with stroke serum Yeon Hee Choa, Eun Hee Kima, Dong Hee Kimb, Ji Hee Sunga, Mi Jeong Oha, Eun Kyoung Shina and Oh Young BangaaIRCCS Istituto Ortopedico Galeazzi, Milano, Italy; bDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milano, Italy; cDepartment of Biomedical, Surgical and Dental Sciences, University of Milan. IRCCS Istituto Ortopedico Galeazzi, Milano, ItalySamsung Health-related Center, Seoul, Republic of Korea; University, Seoul, Republic of KoreabSungkyunkwanIntroduction: Serum from stroke patients increases mesenchymal stem cells trophism towards the infarctedIntroduction: Up to now a number of clinical trials have shown the safety and efficacy of the intra-articular injection of Adipose-derived Mesenchymal Stem/ Stromal Cells (ASCs) in contrasting osteoarthritis.ISEV2019 ABSTRACT BOOKSince ASCs act predominantly via paracrine mechanisms, their secretome represents a promising cell-free alternative. Right here we identified anti-hypertrophic and anti-catabolic effects of ASC conditioned medium (ASC-CM) on TNF-stimulated human major articular chondrocytes (CHs). Approaches: CHs have been treated with ten ng/mL TNF and/ or ASC-CM administered at a 1:five recipient:donor cell ratio. Cell viability was assessed up to day 9. The activity, expression and/or release of hypertrophy markers (MMP-13, Collagen X and Osteocalcin), catabolic mediators (MMP-3) and cartilage-protective elements have been assessed as much as day three by enzymatic assays, qRTPCR, Western Blot and multiplex immunoassays. Outcomes: ASC-CM blunted TNF-induced hypertrophy, minimizing the enhanced levels of MMP-13 activity (-61), SIRT1 MedChemExpress Osteocalcin (-37) and Collagen X (-18). Furthermore, also MMP-3 activity was diminished by -59.