Fri. Jun 21st, 2024

7.30; found: C, 82.78, H, 7.31 . Methyl 2,three,4-tri-O-cinnamoyl-6-O-myristoyl–Dgalactopyranoside (eight). FTIR (KBr) (max): 1702 (-CO) cm-1. 1H-NMR (CDCl3, 400 MHz) ( ppm): H 7.75 7.52, 7.37 (3 1H, three d, J = 16.0 Hz, three PhCH = CHCO-), 7.54 (6H, m, Ar ), 7.28 (9H, m, Ar ), six.55, 6.16, 6.07 (three 1H, 3 d, J = 16.1 Hz, three PhCH = CHCO-), 5.48 (1H, d, J = eight.two Hz, H-1), five.34 (1H, dd, J = eight.two and ten.six Hz, H-2), 5.05 (1H, dd, J = 3.two and ten.six Hz, H-3), 4.66 (1H, d, J = three.7 Hz, H-4), 4.40 (1H, dd, J = 11.2 and 6.six Hz, H-6a), four.01 (1H, dd, J = 11.2 and 6.8 Hz, H-6b), three.52 (1H, m, H-5), three.50 (3H, s, 1-OCH3), two.32 2H, m, CH 3(CH 2) 11CH 2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.25 20H, m, CH3(CH2)10CH2CH2CO-, 0.88 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 795.97. Anal Calcd. for C48H58O10: C, 72.52, H, 7.35; identified: C, 72.53, H, 7.37 .Methyl 6-O-myristoyl-2,3,4-tri-O-(p-toluenesulfonyl)–Dgalactopyranoside (9). FTIR (KBr) (max): 1705 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz) ( ppm): H eight.03 (three 2H, m, Ar ), 7.94 (three 2H, m, Ar ), five.23 (1H, d, J = eight.two Hz, H-1), five.08 (1H, dd, J = eight.0 and 10.5 Hz, H-2), four.77 (1H, dd, J = three.1 and ten.six Hz, H-3), four.53 (1H, d, J = three.7 Hz, H-4), 4.27 (1H, dd, J = 11.0 and six.five Hz, H-6a), 4.11 (1H, dd, J = 11.1 and 6.eight Hz, H-6b), 3.98 (1H, m, H-5), 3.46 (3H, s, 1-OCH3), two.37 2H, m, CH3(CH2)11CH2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.27 20H, m, CH3(CH2)10CH2CH2CO-, 0.98 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 868.ten. Anal Calcd. for C42H58O13S3: C, 58.17, H, six.74; identified: C, 58.19, H, six.76 . Methyl two,three,4-tri-O-(3-chlorobenzoyl)-6-O-myristoyl-D-galactopyranoside (10). FTIR (KBr) (max): 1709 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz): H eight.05 (3H, m, Ar ), 7.96 (3H, m, Ar ), 7.55 (3H, m, Ar ), 7.38 (3H, m, Ar -H), 5.63 (1H, d, J = 8.1 Hz, H-1), 5.21 (1H, dd, J = 8.two and ten.6 Hz, H-2), 5.01 (1H, dd, J = 3.1 and ten.6 Hz, H-3), 4.65 (1H, d, J = 3.7 Hz, H-4), four.40 (1H, dd, J = 11.1 and 6.6 Hz, H-6a), four.20 (1H, dd, J = 11.2 and 6.eight Hz, H-6b), four.00 (1H, m, H-5), three.46 (3H, s, 1-OCH3), 2.35 2H, m, CH3(CH2)11CH2CO-, 1.65 2H, m, CH3(CH2)10CH2CH2CO-, 1.24 20H, m, CH3(CH2)10CH2CH2CO-, 0.86 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 821.19. Anal Calcd. for C42H49O10Cl3: C, 61.50, H, six.02; identified: C, 61.52, H, six.03 .Antimicrobial screeningThe fifteen modified thymidine derivatives (20) have been subjected to antibacterial screening applying 5 bacterial strains: two Gram-positive strains, namely, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538, and three BRD2 Compound Gram-negative strains, namely, Escherichia coli ATCC 8739, Salmonella abony NCTC 6017 and Pseudomonas aeruginosa ATCC 9027. Each of the compounds had been dissolved in dimethylformamide (DMSO) to obtain a 2 option (w/v). In addition, antifungal activities of the compounds have been studied against two fungi strains, namely, Aspergillus niger ATCC 16,404 and Aspergillus flavus ATCC 204,304. These test micro-organisms (bacteria and fungi) were obtained from the Department of Microbiology, University of Chittagong, Bangladesh. Disks soaked in DMSO were applied because the negative manage.Screening of antibacterial activityThe antibacterial spectra with the test derivatives have been obtained in vitro by the disk diffusion strategy [29]. This process applied paper disks of four mm diameter along with a glass AMPK Biological Activity Petri-plate of 90 mmGlycoconjugate Journal (2022) 39:261diameter throughout the experiment. Sterile five (w/v) dimethyl sulfoxide (DMSO) option prepared the synthesized compounds’ desired concentration and normal antibiotics. The pa