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Recently, numerous research have discovered that meniscal tears, degeneration, andFrontiers in Genetics | frontiersin.orgOctober 2021 | Volume 12 | ArticleJiang et al.Osteoarthrititc Meniscus Expression Profilestotal meniscectomy are of high relevance using the onset of osteoarthritis (OA), a common chronic illness that largely happens inside the knee joint (Berthiaume et al., 2005; Hunter et al., 2006; Murphy et al., 2019). The Bax drug possible “meniscal pathway” to knee OA could be attributed to abnormal biomechanical anxiety triggered by meniscal trauma or dissection (Englund et al., 2012). Nevertheless, small analysis has been performed to investigate the precise mechanism underlying meniscal pathology and OA. Hence, it is actually essential to study particular meniscal degenerative mechanisms to cope with all the diagnosis and therapy of early OA. To study the potential regulating mechanism amongst transcriptional and post-transcriptional modification, bioinformatics has been broadly applied. Numerous novel procedures have already been used to demonstrate the mechanisms responsible for several diseases, by way of example, using the use of whole-transcriptome sequencing, with which Lei et al. were in a position to uncover the extensive circular RNA (circRNA) profile of peripheral blood mononuclear cells in hepatocellular carcinoma (HCC) individuals and identified circ_0000798 as a characteristic biomarker for HCC sufferers (Lei et al., 2019). In orthopedic fields, whole-transcriptome sequencing (Li et al., 2019) and single-cell sequencing (Ji et al., 2019) on human principal OA chondrocytes also assisted together with the understanding from the degenerative mechanism of cartilage. Preceding research have also screened out the prospective messenger RNA (mRNA), CCR3 Biological Activity microRNA (miRNA), and long noncoding RNA (lncRNA) in knee cartilage, which might possess cartilage degeneration in the course of OA process (Chen and Chen, 2020; Qi et al., 2020). Lately, using the aid of single-cell sequencing, we had been capable to identify typical and degenerative meniscus cell kinds and superficially uncovered that the transition from a meniscus fibrocartilage progenitor (FCP) cell to a meniscus degenerative progenitor cell (DegP) is possibly the important figuring out issue in the OA meniscus (Sun et al., 2020). Nonetheless, the molecular mechanism underlying this transition remains unknown. In this study, we performed whole-transcriptome sequencing on degenerative meniscus with or without having interleukin-1 (IL-1) therapy as inflammatory chemokines, which includes IL-1, happen to be studied as necessary catabolic mediators in OA which are also applicable to the meniscus (McNulty et al., 2013; Cook et al., 2018). Our previous study has also shown that with 48-h IL-1 (5 ng/ml) therapies, the amount of FCP cells decreased while DegP improved, possibly causing the transition from FCP to DegP (Sun et al., 2020). Therefore, by utilizing IL-1 as an OA inducer in meniscus, we were able to examine the mRNA, miRNA, lncRNA, and circRNA expression profiles in degenerative menisci to identify the characteristics of transcriptional and post-transcriptional variations through the OA degenerative approach. Furthermore, we overlapped three databases of degenerative menisci to select very specific biomarkers in the meniscus for diagnosing early-stage OA, such as our prior single-cell sequencing on normal menisci and OA degenerative menisci (Sun et al., 2020), whole-transcriptome sequence of IL1-abundant menisci, and RNA-seq of control menisci at the same time as OA degenerative meniscus.Components