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Way have been identified by KEGG enrichment annotation (Fig. 4: five). KEGG Na+/Ca2+ Exchanger Purity & Documentation analysis showed
Way had been identified by KEGG enrichment annotation (Fig. 4: 5). KEGG analysis showed that compared with CAK (BR spraying for 0 h), the expression in the UTPglucose-1-phosphate uridylyltransferase (UGP), SPS, glucose-6-phosphate isomerase (GPI), pyrophosphateJin et al. BMC Genomics(2022) 23:Page ten ofFig. 5 A doable model of your BR RGS Protein list signaling pathway with BRs (the activation state of BR signaling) sprayed onto tea leavesJin et al. BMC Genomics(2022) 23:Web page 11 offructose-6-phosphate 1-phosphotransferase (PFP), and epidermis-specific secreted glycoprotein (EP) key regulatory genes related towards the sucrose biosynthesis pathway have been upregulated immediately after BR spraying for three h, 9 h, 24 h, and 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes in the biosynthetic pathway of flavonoidsEleven genes involved in flavonoid biosynthesis were identified by KEGG enrichment annotation (Fig. four: six). The flavonoid biosynthesis-related genes PAL, C4H, 4CL, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3,5-hydroxylase (F3’5’H), DFR, LAR, ANR, and UFGT have been upregulated, with peak values observed at 48 h.DiscussionBR signal transduction mechanism in tea leavesThrough KEGG enrichment and annotation, 26 genes involved in the BR signal transduction pathway have been identified. In line with the heat maps of genes associated to BR signal transduction below diverse BR treatment options, it was identified that 26 genes in the BR signal transduction pathway were considerably upregulated with rising BR spraying time. Combined with the BR signal transduction maps of Arabidopsis and rice, we describe a possible model in the BR signal pathway in tea leaves [291] (Fig. five). At present, the signal transduction pathway of BR in Arabidopsis and rice has been reported. Compared with rice, the signal transduction pathway of BR in tea leaves is equivalent to that of Arabidopsis [24]. In contrast to the BR signal transduction pathway within a. thaliana, BAK1-like kinase contains both SERK and TMK4 inside the BR signal transduction pathway of tea leaves. In our transcriptome data, the ATBS1-interacting aspects (AIF) and paclobutrazol resistance 1 (PRE) genes didn’t considerably differ in expression levels, whereas that in the teosinte branched (TCP) gene was considerable. AIF is definitely the unfavorable regulator of BR signal transduction, even though PRE and TCP would be the positive regulators of BR signal transduction [34]. The outcomes showed that TCP, the forward regulator of BR signal transduction, plays a leading role within the effects from the exogenous spraying of BRs onto young tea leaves.Exogenous spraying of BR promotes the development and development of tea plantsGBSS, and SBE genes associated to starch synthesis; plus the flavonoid biosynthesis-related PAL, C4H, 4CL, CHS, CHI, F3H, F3’5, DFR, LAR, ANR, and UFGT genes had been identified. The outcomes showed that exogenous spraying of BRs upregulated the expression of genes associated to sucrose synthesis, chlorophyll synthesis, starch synthesis, and flavonoid biosynthesis. It might be inferred that exogenous BR spraying improved the content of sucrose, chlorophyll, starch, and flavonoids. Furthermore, a large quantity of hugely expressed cyclin genes, including Cyc, CycD3, CycD4, and CDC6, had been identified. Cell cycle regulatory proteins can bind to cell differentiation cycle-coding proteins and activate corresponding protein kinases, thus advertising cell division. BRs can enhance plant growth by promoting cell division.