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Or car. In either case C60 exposure exacerbated myocardial infarction. Inside each and every delivery route, infarct sizes within the male groups had been bigger than these in females. Among delivery routes, the females had bigger infarctions in response to IT C60 exposure compared with IV exposure. p 0.05 by two-way ANOVA, N = four?.TLR2 Antagonist Synonyms exposed to IV C60 when compared with serum collected from male rats exposed to IV car and male rats exposed to IT C60 (Fig. 4A). Female rats exposed to IV C60 had substantially reduce serum IL-6 concentrations than IV C60 exposed males. Serum IL-6 concentrations were unaltered among all IT groups and IV exposed females. MCP-1 showed a related profile to IL-6. MCP-1 concentrations were greater in serum-collected post-I/R from male rats exposed to C60 IV when compared with serum collected from male rats exposed to IV vehicle, male rats exposed to IT C60 and female rats exposed to C60 IV (Fig. 4B). Female rats exposed to IV C60 had considerably lower serum MCP1 concentrations than IV C60 exposed males. Serum MCP-1 concentrations were unaltered amongst all IT groups and IV exposed females. VEGF was variable and slightly NUAK1 Inhibitor Synonyms elevated in females exposed to IT and IV C60 , although not substantially dif-TABLE 2 Rat Pulmonary Responses to Intratracheal (IT) or Intravenous (IV) Delivery of C60 or Automobile SuspensionsTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in males, information expressed as mean ?SEM Protein ( g/ml) IT Car 560 ?149 C60 316 ?55 Na�ve i 456 ?31 n 4? p 0.05 versus IT C60. IV 638 ?100 750 ?138 Cell number (?105 ) IT 5.7 ?1.4 three.9 ?0.8 5.6 ?0.5 4? IV three.5 ?0.9 six.9 ?1.7 Macrophages (?105 ) IT five.3 ?1.3 4.0 ?0.7 five.six ?0.five three? IV five.7 ?0.7 two.5 ?1.two Neutrophils (x 103 ) IT 14.2 ?10 13.3 ?12 7.7 ?four.two three? IV two.1 ?0.3 1.7 ?1.4 Eosinophils (?03 ) IT 1.9 ?1.1 0.5 ?0.five 1 three? IV two.4 ?two.four 1 Epithelial cells (?103 ) IT six.6 ?two.four two.8 ?0.three 8.7 ?4.0 three? IV 1.7 ?1.7 8.two ?two.six CARDIOVASCULAR INJURY IN RESPONSE TO CTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in females, data expressed as imply ?SEM Protein ( g/ml) Automobile C60 Na�ve i n IT 252 ?28 206 ?24 IV 195 ?eight.2 226 ?41 N/A five? Cell number (?105 ) IT IV 7.eight ?1.9 4.1 ?0.6 7.1 ?1.2 3.five ?0.five 6.6 ?1.0 4? Macrophages (?105 ) IT IV 1.six ?0.9 three.3 ?0.4 0.9 ?0.three 3.1 ?1.1 6.six ?1.1, 4? Neutrophils (?103 ) IT 35.0 ?25.three 27.5 ?11.1 IV 1 1 Eosinophils (?103 ) IT IV 0.25 ?0.25 1.7 ?1.7 12.5 ?7.5 3.six ?0.six 1 four? Epithelial cells (?103 ) IT IV 132 ?90 346 ?132 75.0 ?33 362 ?59 179 ?43 4?1 four?N/A, not applicable. p 0.05 versus IT C60 . p 0.05 versus IT automobile.THOMPSON ET AL.TABLE 3 Rat Coronary Artery Pharmacological Responses for Serotonin (5-HT), ACh, SNP, and ET-Male rat EC50 s [nM] (mean ?SEM) IT delivery C60 5-HT ACh SNP ET-1 559 ?104 265 ?45 116 ?24 38 ?20 Vehicle 871 ?130 239 ?34 76 ?18 48 ?15 IT delivery 5-HT ACh SNP ET-1 C60 1.7 ?0.1 two.6 ?0.four two.4 ?0.six 1.3 ?0.2 Automobile 3.2 ?0.7 1.7 ?0.1 2.two ?0.3 1.0 ?0.05 IT delivery 5-HT ACh SNP ET-1 C60 813 ?128 1419 ?843 19 ?three 29 ?20 Car 1268 ?497 139 ?28 21 ?4 6.five ?2.four IT delivery 5-HT ACh SNP ET-1 C60 1.4 ?0.four 1.0 ?0.four 1.6 ?0.three 1.4 ?0.3 Car 1.0 ?0.2 1.6 ?0.3 2.1 ?0.4 1.1 ?0.3 p-value, n 0.39, five 0.29, five 0.35, 4 0.41, 5 C60 1.3 ?0.four 1.0 ?0.2 two.0 ?0.three 1.1 ?0.two p-value, n C60 0.10, 4 two.0 ?0.two 0.ten, 4 3.8 ?0.8 0.77, 4 two.1 ?0.3 0.09, 6 1.1 ?0.1 Female rat EC50 s [nM] (imply ?SEM) p-value, n 0.06, 4 0.33, 4 0.13, 4 0.49, 6 C60 IV deliv.