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Original ArticleDistribution of Class I Integron and smqnr Resistance Gene
Original ArticleDistribution of Class I Integron and smqnr Resistance Gene Amongst Stenotrophomonas maltophilia Isolated from Clinical Samples in IranMohammadali Malekan 1, Bahman Tabaraie 2 , Ladan Akhoundtabar three, Parviz Afrough 1,four, and Ava Behrouzi1. Microbiology Analysis Center, Pasteur Institute of Iran, Tehran, Iran 2. Kousha Faravar Giti, Industrial Research Institute of Biotechnology, Tehran, Iran 3. Division of Microbiology, Azad University of Jahrom, Jahrom, Iran 4. Department of Mycobacteriology and Pulmonary Investigation, Pasteur Institute, Tehran, IranAbstractBackground: Stenotrophomonas maltophilia (S. maltophilia) can be a multiple-antibioticresistant opportunistic pathogen that is getting isolated with increasing frequency from sufferers with health-care-associated infections. S. maltophilia is inherently resistant to many of the obtainable antimicrobial agents. Spread of resistant strains has been attributed, in part, to class I integrons. In vitro susceptibility studies have shown trimethoprim-sulfamethoxazole and new floroquinolones as two vital agents with activity against these organisms. Strategies: 150 isolates of S. maltophilia were isolated from clinical samples such as respiratory discharges, sputum, and catheter and hospital environments. These isolates have been also subjected to susceptibility testing and polymerase chain reaction for four groups of genes such as int encoding integron components, sulI and sulII encoding trimethoprim-sulfamethoxazole resistance and smqnr encoding quinolone resistance. Outcomes: The price of resistance to trimethoprim-sulfamethoxazole was as much as 27 (18 ) along with the highest resistance to quinolone family members belonged to ofloxacin (20 ) along with the lowest price was for gatifloxacin (16 ). The results showed that 14 of isolates contained integron elements concomitantly with sulI and sulII genes. Conclusion: Resistance rate of S.MFAP4 Protein medchemexpress maltophilia to co-trimoxazole and fluoroquinolones and detection of integron elements between isolates in this study showed that this rate corresponded to other data obtained from other research.NKp46/NCR1, Mouse (HEK293, Fc) Avicenna J Med Biotech 2017; 9(3): 138- Corresponding author: Parviz Afrough, Ph.PMID:22943596 D., Pasteur Institute of Iran, Tehran, Iran Tel: +98 21 66953311 Fax:+98 21 66492619 E-mail: afroughparviz9@gmail Received: 24 Jul 2016 Accepted: 17 OctKeywords: Resistance, Stenotrophomonas maltophilia, Trimethoprim-sulfamethoxazoleIntroduction Stenotrophomonas maltophilia (S. maltophilia) is definitely an aerobic, nonfermentative, gram-negative, catalase-positive and oxidase-negative bacterium. S. maltophilia is ubiquitous in aqueous environments such as water, urine, or respiratory secretions, soil and plants 1. This bacterium causes nosocomial infections in immunocompromised patients and regularly colonizes breathing tubes which include endotracheal or tracheostomy tubes, the respiratory tract and urinary catheters. Infection effortlessly commences by the presence of prosthetic material (plastic or metal), and also the most helpful therapy is removal of the prosthetic devices. Therefore, development of S. maltophiliaisolated from respiratory or urinary specimens in microbiological media is hard to interpret and not a proof of infection. Having said that, isolation of S. maltophilia from sterile physique regions (e.g., blood) typically represents true infection. In immunocompetent folks, S. maltophilia is actually a fairly unusual causeof pneumonia, urinary tract infection, or blood stream infection. S. maltophilia is naturally resistant to.